Addition of RE sites and fusion proteins.
March 23, 2020
Explaining the workings of nature, origins, and/or the meaning of life and death
March 23, 2020
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Addition of RE sites and fusion proteins.

Describe how the PCR can be used to add restriction sites to DNA and explain the cloning procedure. Show how PCR primers are designed and give examples using a real cDNA sequence. Show how PCR is used to make N- and C-terminal fusion proteins. Use expression vectors containing an N- and C-terminal His-Tag.

Sample Solution

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