– N2O will produce as a response to NO toxicity in N.europaea.
– Removing NO via PTIOs from the media will decrease the NO2- toxicity on N.briensis .
-To study the role of NO via removing it by PTIOs and addition 10 mM NO2- concentration on production of N2O in N. europaea, also, in N. briensis to test the NO2- concentration effect alone.
-To compare the role of NO by removing it on the culture of N. europaea and N. briensis without addition NO2-.
topic is about the role of NO toxicity in production of N2O by ammonia oxidizing bacteria
5.3.1 Effect of different treatments on concentration of N2O
Figure 1 N2O production at different treatments in N. briensis presenting maximum N2O production in control and minimum with augmentation of NO2- (A) N2O production maximum in N.europaea supplemented withNO2-(B)
5.3.2 Effect of removing NO via PTIOs on NO2-production by ammonia oxidizing bacteria
Figure 2 Plot of the average NO2-concentration with and without the use of PTIO along with N.briensis (A). Plot of NO2-concentration with and without the use of PTIOs along with N.europaea (B).Values are means ? one standard error (n= 4).
5.3.3 Theeffect of removing NO via PTIOs on NO2-production by ammonia oxidizing bacteria in the samples containing 10 mM NO2-and comparing them with the samples containing 10 mM NO2-
Figure 3 Plot of NO2- concentration (?M)in N.briensis on y and the time (in days) on x (A). Plot of NO2- concentration (?M) in N.europaeaon y and the time (in days) on X (B).Values are means ? one standard error (n= 4).
5.3.4 Bacterial growth rate as affected by different Treatments in ammonia oxidizing bacteria
Table1 The growth rate of N.europaea and N.briensis at different treatments.
The treatment The growth rate ( d-1 ) Standard errors
N. europaea-PTIO 0.5 0.04
N. europaea+PTIO 0.2 0.02
N. europaea + NO2- 0.05 0.03
N. europaea+ NO2- +PTIO 0.06 0.02
N. briensis-PTIO 0.4 0.03
N. briensis+PTIO 0.4 0.04
N. briensis + NO2- 0 0
N. briensis + NO2- +PTIO 0 0
Figure 4Production of NO in N.europaea at 0 mM and at 10mM NO2- and removing NO from both concentrations by adding PTIO (A). Production of NO in N.briensis at 0 mM and at 10mM NO2- and removing NO from both concentrations by adding PTIO (B). Values are means ? one standard error (n= 4).